BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: X10+X9GAX9 phage display library pool

Library ID:

205

Library Length:

10,18

Library Complexity:

2e8, 4e8

Library Titer:

Library From:

De Ciechi

Library Randomness:

Semi-random

Library Scheme:

Library Topology:

Linear

Comments:

The 10-mer and 20-mer linear library were constructed with vector pMON6000 and pMON20401 respectively. Both vectors were derived from the fUSE3 vector. The peptides are expressed immediately following the gene III signal peptide cleavage site. The codons of the degenerate oligonucleotides used for construction of the libraries followed the formula NNK, where N is an equimolar mixture of all four deoxynucleotides and K is an equimolar mixture of dGTP and dTTP. NH2-X10-GG=TVES; NH2-X9GAX9-GAAGGAGAGAG=TVES......

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