BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: X2CX4-8CX2 phage display library pool

Library ID:

204

Library Length:

10-14

Library Complexity:

3e8

Library Titer:

Library From:

De Ciechi

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

Five constrained libraries wherein random peptides, varying from four to eight amino acids, are flanked by a Cys on either side in addition to a further two random residues on either side were constructed with pMON20401 vector, which was derived from fUSE3 vector. The peptides are expressed immediately following the gene III signal peptide cleavage site. The codons of the degenerate oligonucleotides used for construction of the libraries followed the formula NNK, where N is an equimolar mixture of all four deoxynucleotides and K is an equimolar mixture of dGTP and dTTP. NH2-X2CX4-8CX2-GAAGGAGAGAG=TVES...

First |

Prev |

204

| Next

| Last