BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: XCXmCXnCXoCX phage library pool

Library ID:

528

Library Length:

9-14

Library Complexity:

Library Titer:

Library From:

Harm-Anton Klok (Laboratoire des Polymères, Institut des Matériaux and Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland)

Library Randomness:

Library Scheme:

Library Topology:

Circular

Comments:

Peptide libraries XD9 to XD14 were constructed. The libraries had the peptide format of XCXmCXnCXoCX (m + n + o = 3, 4, 5, 6, 7 or 8; where X indicates random amino acids, C indicates cysteine). The libraries are constructed from a filamentous phage fd, which displays peptides on the N terminus of the pIII coat protein at the end of the viral particle. In total, five peptides are displayed per viral particle. Escherichia coli TG1: All phage display experiments utilized E. coli TG1 cells for phage amplification and titer.

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