BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 931

Peptides:

Sequences

Affinity Values

YGSWAS(2)
YGGLGL(1)
YGSLVL(1)
YGGLGI(1)
YGSLVQ(1)
YGGLGR(1)
YGSLVR(1)
YGGLNV(1)
YGSLAD(1)
YGGLRA(1)
YGSLLS(1)
YGGLEM(1)
YGSLNG(1)
YGSLYE(1)
YGGIAS(1)
YGGIAV(1)
YGGIRP(1)
YGSWQA(1)
YGGWAG(1)
YGGWGP(1)
YGSFLH(1)
YGGWSS(1)
YGGMKV(1)
YGGFPD(1)
YGALGG(1)
YGALSW(1)
YGALDT(1)
YGALEL(1)
YGAIGF(1)
YGAWTR(1)
YGWWGL(1)
YGLWQS(1)
YGWWLT(1)
YGFWGM(1)
YGWLAT(1)
YGKWSG(1)
YGWANK(1)
YGPFWS(1)
YGEFVL(1)
YGNWTY(1)
YGDFAF(1)
YGNFAD(1)
YGNFPA(1)
YAWGWG(1)
YAGFAQ(1)
YGTFIL(1)
YGTWST(1)
YSMFKE(1)
YGVWAS(1)
YGVWWR(1)

[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[7.8]
[NT]
[NT]
[NT]
[NT]
[2.3]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[0.35]
[NT]
[NT]
[NT]
[1.9]
[NT]
[7.8]
[NT]
[NT]
[NT]
[NT]
[8.3]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 2201029

Target Name:

Anti-beta-endorphin monoclonal antibody 3-E7

Template Name:

Beta-endorphin

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X6 fAFF1 phage display library

Affinity Measurement Method:

Radioimmunoassay

Affinity Measurement Description:

A solution radioimmunoassay (RIA) was used to estimate the binding affinities of peptides for mAb 3-E7. Six hexapeptides with sequences corresponding to those of antibody-selected phage were chemically synthesized and assayed for binding to 3-E7. Each Kd (μM) value is the antilogarithm of the mean of log Kd from three to six independent determinations with standard deviations of <0.25 logarithm units in all cases. NT denotes not tested.

Brief Description:

BiopanningDataSet Comments:

Compared with 7.1 nM for a known high-affinity ligand (YGGFL), the binding affinities of six chemically synthesized hexapeptides from this set range from 0.35uM (YGFWGM) to 8.3uM (YAGFAQ).

Curator:

Curation Date:

0000-00-00

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