BDB -BiopanningDataSet Details

BiopanningDataSet
Target
Template
Library
Structure

Biopanning Data

BiopanningDataSet ID: 930

Peptides:

Sequences
IWTWEPSKRYQL

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7536850

Target Name:

Anti-proenkephalin monoclonal antibody PE2

Template Name:

Proenkephalin-A

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X12 fAFF1 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For antibody PE2, the amplified library after the first round was subjected to two further rounds of antibody panning. In each round, 10 ml of library was panned on Petri dishes coated with 10 mg/ml PE2 and subsequently blocked with TBST buffer containing 5% (w/v) low-fat milk powder, for one hour.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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