BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 932

Peptides:

Sequences

WNVHGIWQE(2)
DWDTRGLWVA(2)
WWTDTGLW(1)
WWTDDGLW(1)
WWDTRGLWVWTI(1)
FWGNDGIWLESG(1)
DWDQFGLWRGAA(1)
RWDDNGLWVVVL(1)
CWSMHGLWLC(1)
SGMWSHYGIWMG(1)
GGRWDQAGLWVA(1)
KLWSEQGIWMGE(1)
GCWDNTGIWVPC(1)
DWDTRGLWVY(1)
SLWDENGAWI(1)
KWDDRGLWMH(1)
QAWNERGLWT(1)
QWDTRGLWVA(1)
SWDTRGLWVE(1)
SWGRDGLWIE(1)
EWTDNGLWAL(1)
SWDEKGLWSA(1)
SWDSSGLWMD(1)

Sequences Features:

Motif:

W-x(3)-G-x-W

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3-4

Reference:

PMID: 8693002

Target Name:

Interleukin-1 receptor type 1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X8, X10 and X12 phage display library pool

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Extracellular domain of type I IL-1 receptor was fuse to Fc domain of IgG1. The IL-lR-Fc fusion was then used as target.

BiopanningDataSet Comments:

These peptides blocked binding of IL-la to the type I IL-1 receptor with IC50 values of 45-140uM. They also blocked IL-1-driven responses in human and monkey cells; howver, they did not bind the human type II IL-1 receptor or the murine type I IL-1 receptor.

Curator:

Curation Date:

0000-00-00

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