BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 742

Peptides:

Sequences

Affinity Values

RINNIPWSEAMM(8)
CHPLTPWEC(6)
TSPYEDWQTYLM(5)
FWHPMHD(4)
SWPWDTW(3)
DTHRWPWYISQE(1)
SPWYSDYTRYLW(1)
SDPYKLWASYMY(1)
QPASNMTLGRGL(1)
SSVDESTAQVNW(1)

[0.0087 ± 0.0021, 0.0061 ± 0.0016, 0.0094 ± 0.0005][-8.90]
[>500, >500, >500][383.90]
[>500, >500, >500][463.14]
[>500, 68 ± 4, >500][94.28]
[>130, 21, ND][74.36]
[500, 259 ± 12, 348 ± 3][88.35]
[>140, >140, ND][373.94]
[>100, >100, ND][528.60]
[NT][NT]
[NT][NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10364331

Target Name:

Envelope glycoprotein gp120 (SU)

Template Name:

Anti-gp120 monoclonal antibody 17b

Structure of Target-Template Complex:

1G9M

1G9N

1GC1

1RZJ

1RZK

2NXY

2NXZ

2NY0

2NY1

2NY2

2NY3

2NY4

2NY5

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12, Ph.D.-7 and Ph.D.-C7C phage display library pool

Affinity Measurement Method:

ELISA,Surface plasmon resonance (SPR)

Affinity Measurement Description:

The inhibitory capacities of the identified peptides were tested with gp120 from an additional T-tropic strain (HXB2 and SF2) and an M-tropic strain (ADA). IC50s (μm, means ± standard errors) for inhibition of the 4dCD4-gp120 interaction was measured by competition ELISA. NT, not tested. ND, not determined. Besides, surface plasmon resonance (SPR) binding assays were performed on a Biacore (Piscataway, N.J.) instrument. The MAb 17b was coupled to a CM5 sensor chip. MAb 17b was dissolved in 10 mM acetic acid-sodium acetate buffer (pH 4.9). The surface was regenerated twice with 5 ml of 4.5 M MgCl2 after gp120 binding. Sequential injections (20 μl) of HXB2-gp120 (200 nM) with peptide at increasing concentrations (0.2 to 200 μM) were made over a surface with 1,500 RU of coupled MAb 17b. The amount of gp120 bound (expressed as surface plasmon resonance units [RU]) was measured 10 s after the end of the injection and by subtracting RUs bound for the same solution on a blank mock-coupled surface. Results are given as the percentage of gp120 bound relative to gp120 bound in the absence of peptide: 100 + 100 × [(RU - RUgp120)/RUgp120]. The data shown in the second square bracket were reproduced from FIG. 4 in the reference.

Brief Description:

In the first round of selection, bound phage were eluted with glycine of BSA. In subsequent rounds of selection, phage were eluted with solution of gp160(502-516) in PBSTB.

BiopanningDataSet Comments:

RINNIPWSEAMM inhibit the interaction between gp120 and both four-domain soluble CD4 (4dCD4) and monoclonal antibody (MAb) 17b, a neutralizing antibody that covers the chemokine receptor binding surface on gp120.

Curator:

Curation Date:

0000-00-00

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