BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3313

Peptides:

Sequences	Affinity Values
DSSAVCWAFPHHPLCHMKAT ADADMCWFFPTSPWCH DLSAVCWAFPWDPECH DSSAVCWAFPYLPECH DISAVCWAFPFDPECH	[0.15][4.98] [0.92][0.67 ± 0.62] [0.03][0.016 ± 0.013] [0.17][0.048 ± 0.018] [0.25][0.039 ± 0.018]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29329326

Target Name:

Avi-tagged interleukin-17A

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

IX104 phage display library(X5CX8CX5)

Affinity Measurement Method:

ELISA,Surface plasmon resonance (SPR)

Affinity Measurement Description:

BIAcore T200 was used to determine binding affinity and kinetics of the peptides to IL-17A. Avitagged IL-17A was captured to streptavidin sensor chip.For each SPR run, the sensor was first conditioned by 8 cycles of blank prior to sample injections.The resulting sensorgrams of the concentration series of a same peptide were fitted globally to extract binding kinetics and affinity with BIAcore T200 Evaluation Software 2.0 using a 1:1 binding model. Pure peptides from the two libraries were assayed in AlphaLISA format to investigate the potential for disruption of the IL-17A/IL-17RA cytokine-receptor interaction in a biochemical format. In general, the IC50 values determined by AlphaLISA agreed quite closely with the KD values determined through SPR analysis.As was observed in SPR for this family, the differences in AlphaLISA activity between the affinity matured peptides were not significant, with several peptides having IC50's in the 0.2 to 2.0 μMrange.

Brief Description:

IX104 phage display library(X5CX8CX5) consisting of 18 random amino acids were selected against 100 nM of Avi-tagged IL-17A. After three rounds of selection were completed, the eluted phage pool was deconvoluted by filter-lift.

BiopanningDataSet Comments:

Curator:

Zhongjie Tang

Curation Date:

2018-04-19

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