BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3301

Peptides:

Sequences	Affinity Values
YSLHNAGPWSLQ	[0.29]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29433534

Target Name:

Immunoglobulin G(IgG) of patients with Rheumatoid arthritis-Secondary Sjögren’s syndrome(RA-sSS)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Antibody reactivity against peptides was measured using a solid-phase ELISA and the color intensity was measured at 450 nm.

Brief Description:

BiopanningDataSet Comments:

Phage clones with the sequence YSLHNAGPWSLQ specifically bound to the IgGs of patients with Rheumatoid arthritis-Secondary Sjögren’s syndrome(RA-sSS), which was confirmed by three rounds of screening with the same pooled IgGs.

Curator:

Juanjuan Kang

Curation Date:

2018-04-19

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