BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3262

Peptides:

Sequences
DRWVARDPASIF(39/60) FPSSPGVSRAIN(20/60)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28823053

Target Name:

Macrophage-stimulating protein receptor, MSP receptor

Template Name:

Hepatocyte growth factor-like protein

Structure of Target-Template Complex:

4QT8

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

A subtractive biopanning was first performed by adding a peptide-displaying phage library to pre-cold MDCK cells. Subsequently, the supernatant was transferred onto pre-cold MDCK-RON cells and incubated. Totally, five rounds of biopanning were carried out using MDCK-RON cell line. Then,the recovered phage from fifth round were used for subtractive biopanning using EBC-1-Dox+ (RON negative) in order to eliminate possible non-RON binding phage particles.

BiopanningDataSet Comments:

Curator:

Zhongjie Tang

Curation Date:

2017-10-24

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