BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3261

Peptides:

Sequences

Affinity Values

DRWVARDPASIF(22/60)
FPSSPGVSRAIN(3/60)
GLHTSATNLYLH(2/60)
GVAMLQSGYASR(2/60)
VTVELQHLSPAK(2/60)
FEHSLYKEMTHL(1/60)
NYPATNTHRYTP(1/60)
TPQSFWQKGSLV(1/60)
AFDTFNLYMDEL(1/60)
VPGLVFSKLTTG(1/60)
VPAQSGQGIEHE(1/60)
SPDLSTRPTLGY(1/60)
IPAPLLVPNLWH(1/60)
SSDSVVKQAILT(1/60)
HMNTHDSLFPVG(1/60)
QPNNILPAFGNY(1/60)
APTLYWNVPTHV(1/60)
LFAAASSNHIHR(1/60)
SVLPLLCCDSTP(1/60)
VERSFLNERVTP(1/60)
DVTRLLSTHMWI(1/60)
GIQNGLAFQGLQ(1/60)
QLQLNNQVALMY(1/60)

[0.36]
[0.45]
[1.79]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28823053

Target Name:

Macrophage-stimulating protein receptor, MSP receptor

Template Name:

Hepatocyte growth factor-like protein

Structure of Target-Template Complex:

4QT8

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Brief Description:

A subtractive biopanning was first performed by adding a peptide-displaying phage library to pre-cold NIH3T3 cells. Subsequently, the supernatant was transferred onto pre-cold NIH3T3-RON cells and incubated. Totally, five rounds of biopanning were carried out using NIH3T3-RON cell line. Then,the recovered phage from fifth round were used for subtractive biopanning using EBC-1-Dox+ (RON negative) in order to eliminate possible non-RON binding phage particles.

BiopanningDataSet Comments:

Binding modes and affinities were also predicted by docking and molecular dynamics (MD) simulation. The results of ELISA experiment showed that P6 peptide (FEHSLYKEMTHL) displaying phage has higher affinity for RON compared to others and its binding site is located out of ligand binding site. Docking and MD simulation results also indicated higher affinity of P6 to RON as well as its exosite-binding feature. Taken together,our data suggest a capacity for P6 peptide to be utilized as RON binding agent,and hence be used for various purposes,including design of drug delivery systems for transferring cytotoxic agents to RON-positive cancer cells, interfering with RON signaling, peptidomimetics design, and diagnostic imaging.

Curator:

Zhongjie Tang

Curation Date:

2017-10-24

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