BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3211

Peptides:

Sequences
SGNNPLHVHHDKR

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28578326

Target Name:

T lymphocyte leukemia cell line Jurkat

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

By fusing the Jurkat-binding peptide (SGNNPLHVHHDKR) to the C-terminus of IFNα, we constructed an engineered chimeric IFNα molecule (IFNP) for the treatment of adult T-cell leukemia/lymphoma (ATL). We found that IFNP exhibited significantly higher activity than wild type IFNα in inhibiting the growth of leukemia cells and inducing cell blockage at the G0/G1 phase. The synthetic IFNP molecule exerted its antitumor activity by upregulating the downstream genes involved in the STAT1 pathway and in apoptosis. Using a cell receptor binding assay, we showed that this Jurkat-binding peptide facilitated the binding affinity of IFNα to the cell surface type I IFN receptor. The engineered IFNP molecule may prove to a novel antitumor approach in the treatment of patients with ATL.

Curator:

Min Yu

Curation Date:

2017-07-22

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