BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3212

Peptides:

Sequences	Affinity Values
CNYCRLNLW(19) CRSTLQHSC(1) CLKNQSDQC(1) CSTSSRTGC(1) CMHVRHGLV(1) CDSAPTRKC(1)	[0.36 ± 0.07] [0.27 ± 0.05] [0.20 ± 0.04] [0.04 ± 0.01] [0.16 ± 0.04] [0.16 ± 0.04]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Rounds of Panning:

Reference:

PMID: 28584144

Target Name:

Nitrite reductase AniA

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Phage clones were purified and tested separately to measure their affinity to sAniA in the phage ELISA assay. Absorbance at 450 nm was measured. Readings were compared to wells, which underwent identical treatment but lacked sAniA (control) and to signal from a wild type phage, M13KE (of 1010 PFU), that does not display peptides (wild type). The mean and SEMs from seven independent experiments are shown. Data shown were re-generated from Figure 3C in the reference.

Brief Description:

Magnetic Ni-NTA bead based affinity capture was used to immobilize sAniA. A preclearance step was to remove Ni2+ and plastic binders from the phage pool.

BiopanningDataSet Comments:

Curator:

Min Yu

Curation Date:

2017-07-22

First |

Prev |

3212

| Next

| Last