BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3210

Peptides:

Sequences	Affinity Values
VHWDFRQWWQPS	[1.75 ± 0.12]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28572662

Target Name:

Polystyrene 96-well microtiter plate (polystyrene, PS)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of the positive phage clones to polystyrene were measured by phage ELISA. The OD450nm values at the concentration of TBS with 0.20% (w/v) NFM were measured. Data shown were re-generated from the Figure 2 in the reference.

Brief Description:

BiopanningDataSet Comments:

Peptide VHWDFRQWWQPS, PB-TUP, has been isolated on target proteins (folate receptor-α, programmed death 1 and programmed death-ligand 1). ELISA and phage recovery assay demonstrated that PB-TUP phage had a significant superior affinity to polystyrene solid surface compared with control phage clones. Propagation rate assays of the selected phage clones showed that the growth rate of PB-TUP phage was not superior to the control phages. Furthermore, the binding of PB-TUB to polystyrene was concentration dependent and varied with solution pH. Molecular modeling revealed that stable structures of α-helix and β-turn may contribute to the binding of PB-TUP to polystyrene plate. The PB-TUP sequence was fused to the N-terminus of peptide P2 and the fusion peptide significantly increased the binding affinity to polystyrene. The fusion peptide also enhanced the cell adhesion ability of peptide P2 with human umbilical vein endothelial cell (HUVEC). The addition of the polystyrene binding peptide provided a convenient method for peptide immobilization.

Curator:

Min Yu

Curation Date:

2017-07-22

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