BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3068

Peptides:

Sequences

VPGWSQAFMALA(21)
DTDWVRMRDSAR(9)
DMTYMERRDSAR(2)
NHVPQHTPIHLR(2)
AHVPQHHAMTGR(2)
VPGWSQTFMPLA(1)
GHVTQHSQRGYS(1)
GHVPQHARLLGL(1)
THVPEHLLKPRP(1)
GHVQQHDVHSIR(1)
YHVPEHAVRLGP(1)
KHVLQHQTSMTM(1)
KHVVQHEYAPNA(1)
HHTDEHWLFAKK(1)
LSHRTHDRIGFT(1)
YPPQERTVHKYV(1)
DAYTQLRDRMRQ(1)
IDGYPGSPRLPW(1)
WHWTWPADVGWV(1)

Sequences Features:

Motif:

H-V-P-[GQ]-[WH]-S-Q, D-T-x(4)-R-D

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger Sequencing

Rounds of Panning:

Reference:

PMID: 27659529

Target Name:

Anti-EGFR monoclonal antibody panitumumab

Template Name:

Epidermal growth factor receptor (EC:2.7.10.1)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In each round of selection, phage clones were preabsorbed by human IgG immobilized on rProtein A sepharose beads to remove unspecific phages, followed by positive selection with panitumumab counterpart.

BiopanningDataSet Comments:

To enhance the immune responses, we generated recombinant proteins of P19 (DTDWVRMRDSAR) or P26 (VPGWSQAFMALA) fused to a heat-shock cognate protein 70 (Hsc70), and evaluated the efficacy of Hsc70-P19 and Hsc70-P26 as vaccines in vivo. Immunization with Hsc70-P19 or Hsc70-P26 fusion protein stimulated the immune system to produce specific antibodies against peptides as well as EGFR. Moreover, antibodies elicited against mimotopes could induce antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and inhibit the proliferation of EGFR-overexpressing A431 cells. Treatment with Hsc70-P19 and Hsc70-P26 significantly reduced tumor growth in BALB/c transplantable lung cancer models. Although there was no sequence homology between the phage-derived peptides and EGFR by alignments, both peptides mimic the conformational structure of EGFR binding to panitumumab.

Curator:

Curation Date:

2017-01-12

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