BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3067

Peptides:

Sequences
RCQYPLCS(14/40) SCKTVFCY(3/40) RCLRSHCG(3/40) RCPRFSCW(2/40) SCFRPTCP(2/40) RCQYSPCH(1/40)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger Sequencing

Rounds of Panning:

Reference:

PMID:

Target Name:

Fluorescent phosphor LaPO4:Ce3+,Tb3+ (LAP)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

LX-4 phage display library (XCX4CX)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

A phage clone containing the surface peptide loop RCQYPLCS was found to bind specifically to LAP. Specificity and affinity of the identified phage bound peptide was confirmed by using binding and competition assays, immunofluorescence assays and zeta potential measurements. Binding and immunofluorescence assays identified the peptide’s affinity for the fluorescent phosphor components CAT and BAM. No affinity was found for other fluorescent phosphor components such as YOX. The binding specificity of the RCQYPLCS peptide loop was improved 3-51-fold by using alanine scanning mutagenesis. The identification of peptides with high specificity and affinity for special components in the fluorescent phosphor in CFLs provides a potentially new strategic approach to rare earth recycling.

Curator:

Curation Date:

2017-01-12

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