BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2829

Peptides:

Sequences
SCFQAACWRQLCG(2) NCRAKGCWYGYCF(1) ACRSWSCTCGLCY(1) SCGGDQCALDRCV(1) TCSWGKCQVTDCG(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23100545

Target Name:

Plasma kallikrein (EC:3.4.21.34)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

XCX4CX4CX phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 10 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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