BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2830

Peptides:

Sequences	Affinity Values
ACFDPCRVVCY(1) QCFQWCRTNCY(1) NCWNPCRVACL(1) QCENPCPTSCC(1) TCEKRCCPRCS(4)	[18.2 ± 4.2] [178.3 ± 12.6] [51.7 ± 10.4] [NT] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23100545

Target Name:

Plasma kallikrein (EC:3.4.21.34)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

XCX3CX3CX phage display library

Affinity Measurement Method:

Inhibition assay

Affinity Measurement Description:

Bicyclic peptides were chemically synthesized and their inhibitory activity (IC50, nM) is indicated. NT denotes not tested.

Brief Description:

The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 20 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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