BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2828

Peptides:

Sequences

Affinity Values

GCWPQCRVNCV(3)
TCEKRCCPRCS(3)
KCWQECRSLCY(2)
GCYERCRVYCS(2)
GCYKPCRVNCP(1)
ECFTACRVACI(1)
ECFDKCRVFCV(1)
GCFTPCRTMCW(1)
TCFHHCRVNCS(1)
ACWKLCRVDCV(1)
NCWARCRVMCE(1)
FCFQNCKALCF(1)
SCVRPCFYWCD(1)
SCVDYCVGTCC(1)
LCMDNCEQVCQ(1)
KCGKKCDYRCL(1)

[115.7 ± 24]
[NT]
[NT]
[NT]
[158.7 ± 25.7]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23100545

Target Name:

Plasma kallikrein (EC:3.4.21.34)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

XCX3CX3CX phage display library

Affinity Measurement Method:

Inhibition assay

Affinity Measurement Description:

Bicyclic peptides were chemically synthesized and their inhibitory activity (IC50, nM) is indicated. NT denotes not tested.

Brief Description:

The library was joined and subjected to a first affinity selection with hPK. Isolated phage were amplified and, prior to the second round of affinity selection, treated for 30 min with 10 mg/ml pancreatin. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

2828

| Next

| Last