BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2713

Peptides:

Sequences

Affinity Values

CKSLENSYC(18)
CKSVGNYQC(2)
CKNPTTGTC(2)
CKSLENSQC(1)
CNPSNRNDC(1)
CKSPGNYQC(1)
CKSLEQSYC(1)
CNPSNRQDC(1)
CTGTTNQYC(1)
CNSPRPSTC(1)
CNVQHNKTC(1)
CSTRHTYDC(1)
CIKSTHNHC(1)

[23.4 ± 1.1]
[3.6 ± 0.8]
[4.3 ± 0.3]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24841092

Target Name:

Bisphenol A, BPA

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

The binding specificity and relative affinity of the selected peptides to BPA were compared by assessing the number of BPA bound phages with varying peptide sequences displayed. Wild type M13 phage was used as a control. The number of wild type phages bound to BPA was 2.3 ± 0.2. NT denotes not tested.

Brief Description:

Two rounds of negative panning followed the six rounds of positive screening procedure. The collected phages from the final round of positive panning were incubated in a microcentrifuge tube filled with Binding Buffer lacking BPA for 1 h and centrifuged as above in order to exclude phage particles with cross binding affinity to the tube surface.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

2713

| Next

| Last