BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2714

Peptides:

Sequences
CSMSARQLC(20/25)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24960578

Target Name:

Beta-amyloid protein 42

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Twenty-five Aβ42-binding clones with higher absorbance values were selected and amplified. Twenty clones showed the same peptide sequence: CSMSARQLC. His-tag-conjugated peptide CSMSARQLC bound to Aβ42 in a concentration-dependent manner. The peptide recognized Aβ13–35. In addition, molecular dynamics analysis and ELISA showed that the peptide predominately binds to I31 of Aβ42 by leucine and may also bind to F20 by alanine.

Curator:

Curation Date:

0000-00-00

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