BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2712

Peptides:

Sequences	Affinity Values
RALAHPRDHPDL ATCSMLLSRNEA	[1.25] [1.11]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24779651

Target Name:

Esophageal cancer cell line Eca109

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Phage ELISA was used to test the specific binding of the selected phage clones to Eca109 cells. Normal human esophageal epithelial cells and bovine serum albumin cells served as controls. Absorbance values at 490 nm were subsequently recorded using a plate reader. The data were reproduced from the graph.

Brief Description:

Three rounds of subtractive screening of a phage peptide library were performed using Eca109 as the target cells and normal human esophageal epithelial cells as the absorber cells. For the second and third rounds of selection, the volume of added phage remained the same, yet the incubation time with the Eca109 cells decreased to 45 min and 30 min, respectively. In contrast, the incubation time with the normal esophageal cells increased to 1.25 h and 1.5 h, respectively.

BiopanningDataSet Comments:

While ELISA, immunofluorescence and immunohistochemistry assays were used to validate the binding affinities of two positive phage clones, sequencing of the positive clones did not find any homology between the sequences obtained and a protein database.

Curator:

Curation Date:

0000-00-00

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