BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2496

Peptides:

Sequences
CGLIIQKNEC(3/24) CNAGESSKNC(2/24)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16476999

Target Name:

Human plasma clots

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX8C T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Human plasma clots were incubated with the CX8C peptide library and extensively washed with PBS. Plasma was then added to the clot to remove phage recognizing soluble plasma components.

BiopanningDataSet Comments:

When injected intravenously into mice bearing various types of tumors, fluorescein-conjugated CLT peptides (CGLIIQKNEC and CNAGESSKNC) accumulated in a fibrillar meshwork in the extracellular compartment of the tumors, but were not detectable in other tissues of the tumor-bearing mice. The tumor homing of both peptides was strongly reduced after coinjection with unlabeled CLT2 (CNAGESSKNC), indicating that the two peptides recognize the same binding site. The CLT peptide fluorescence colocalized with staining for fibrin(ogen) present in the extravascular compartment of tumors, but not in other tissues. The CLT peptides did not home to tumors grown in fibrinogen-null mice or in mice that lack plasma fibronectin. The CLT peptides also accumulated at the sites of injury in arteries, skeletal muscle, and skin. It is concluded that the CLT peptides recognize fibrin–fibronectin complexes formed by clotting of plasma proteins that have leaked into the extravascular space in tumors and other lesions. These peptides may be useful in targeting diagnostic and therapeutic materials into tumors and injured tissues.

Curator:

Curation Date:

0000-00-00

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