BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2495

Peptides:

Sequences	Affinity Values
GNVPDMRHIARP IXAKDQNTWPRR VEWPAQRHHHIP YASKFPHLXTLE HLSLPQAPTLLP ADLPSLFASRGT	[420 ± 30] [NT] [NT] [NT] [NT] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 17132933

Target Name:

HIV-1 TAR

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

The association of the purified peptides to TAR was determined by filter binding assay. The dissociation constant KD (nM) value was calculated. NT denotes not tested.

Brief Description:

The library was counter-selected by two successive incubations in the presence of streptavidin coatd beads. Nonbinding phages were recovered and incubated with 3' biotinylated TAR. The mix was then added to streptavidin beads. In the subsequent rounds, competitor yeast tRNA was added.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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