BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2497

Peptides:

Sequences
CHWSFGIFC(8) CHWMFSPWC(3) CPWYFLPWC(2) CPWYFQPWC(2) CPWYFHPYC(1) CPWYFFDLC(1) CPWYFFGAC(1) CSWIFWEYC(1) CSWIFWQYC(1) CSWIFWVYC(1)

Sequences Features:

Motif:

x-W-x-F-x(3)

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3-5

Reference:

PMID: 9094664

Target Name:

Puumala virus

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX7C phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Biopanning with MAb 4G2 as the eluting antibody included three antibody and two acid elution rounds. In the first panning, the wells were rinsed 10 times with 5% NFDMP-TBS, pre-eluted with antibody 5A2, rinsed three times, and eluted with MAb 4G2. The second panning was done directly with MAb 4G2, whereas in the third, MAb3H9 was used as a pre-eluting MAb. For removal of residual antibody, the phage amplification product of the third panning was treated with Prosep-A (Bioprocessing Ltd.). The fourth panning was done conventionally with 0.1 M HCl-glycine, pH2.2, containing 1mg of BSA per ml as the eluent.

BiopanningDataSet Comments:

The peptide insert, CHWMFSPWC, when displayed on the phages, completely inhibited Puumala virus infection in cell culture at the same effective concentration as the eluting antibody specificfor envelope glycoprotein G2.

Curator:

Curation Date:

0000-00-00

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