BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1660

Peptides:

Sequences

ALLSDLYKLL(1)
DSASDLYKLL(1)
ESDLFKMASS(1)
FLYMSDLYKL(1)
HTQFSDLFKL(1)
LMHKARTTWP(1)
LSDLSKLAQS(1)
LSQNGLTSFN(1)
MCNHSDLNKL(1)
MWSDLTKLLP(2)
NMSFSDMWKL(2)
PEMSSDLWKL(1)
PLYSDLLKLL(1)
PQDSDLYKLS(1)
RIWMSDLVKL(1)
RLQDSAVSGH(2)
SDLWKMMDKN(2)
SHRMSDLWKL(1)
SNYVSDLYKL(1)
SVKAWPSAQI(1)
SYTESDLHKL(1)
TLRFSDLIKL(1)
TSSDLWKLLP(2)
TTHSIVSHVS(1)
VYTSSDLNKL(1)
WLVESDLYKL(2)
YSDLDKMKPY(1)
YSDLVKLSTL(1)

Sequences Features:

Motif:

x-S-D-L-x-K-L

Unique Sequence Number:

Experimental Method:

mRNA display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18855146

Target Name:

Anti-p53 monoclonal antibody

Template Name:

Cellular tumor antigen p53

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X10 mRNA display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In order to remove non-specific binders to antibodies, the molecules prepared in the previous section were incubated with human normal IgG (Sigma)-bound Protein G-Sepharose beads (GE Healthcare) for 30 min at room temperature with rotation.

BiopanningDataSet Comments:

Although no peptides that fully matched with hTP53 were found in the clones isolated, the core sequence was found in hTP53. A 10-amino acid peptide containing the core sequence was chemically synthesized to verify its binding with SPR. Its Kd value for the antibody was 6 nM.

Curator:

Curation Date:

0000-00-00

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