BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: X6 M13 phage display library

Library ID:

435

Library Length:

Library Complexity:

6.4e7

Library Titer:

Library From:

Jacek Otlewski (University of Wroctaw, Wroctaw, Poland)

Library Randomness:

Completely random

Library Scheme:

NNS

Library Topology:

Linear

Comments:

The phage-displayed library of random hexapeptides was fused to the C terminus of the M13 phage major coat protein P8, downstream of the AWEENIDSAP linker to ensure optimal C-terminal display of peptide variants on the phage surface. The insert was obtained by hybridization of degenerate and phosphorylated oligonucleotides: bib(for) TCGAGCGGTNSNNSNNSNNSNNSNNSTAATAA and bib(rev) CTAGTTATTASNNSNNSNNSNNSNNSNNACCGC (where N stands for equimolar contribution of A, T, G, C and S for G or C). The resulting cassette with sticky ends (XhoI/SpeI) was inserted into the modified phagemid pComb3H.

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