BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: R26 M13 phage display library (SRX12(A/S/P/T)A(A/D/E/G/V)X12SR)

Library ID:

434

Library Length:

Library Complexity:

2.0e8

Library Titer:

Library From:

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

The library was constructed by annealing and extending two long degenerate oligos with a complementary region at their 3' termini. The 6-nt complementarity corresponded to the SacII recognition sequence and encoded the tripeptide: (A/S/P/T)A(A/D/E/G/V). This design, which fixed Ala as the central aa, permits the subdividing of the long peptides for the analysis of binding residues by SacII digestion of the DNA insert. The library consisted of 2e8 recombinants, each expressing the peptide sequence: SRX12(A/S/P/T)A(A/D/E/G/V)X12SR at the mature N-terminus of pIII.

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