BDB - Library Details

Library

Library Name: FliTrx bacterial display library (X12)

Library ID:

326

Library Length:

Library Complexity:

1.77e8

Library Titer:

2.0e10

Library From:

Invitrogen, Carlsbad, CA

Library Randomness:

Completely random

Library Scheme:

Library Topology:

Linear

Comments:

The FliTrx(TM) Random Peptide Display Library is an E. coli-based system that eliminates the use of phage and makes screening of peptide interactions faster and easier. The FliTrx(TM) Library was constructed in the pFliTrx(TM) vector. A diverse library of random dodecapeptides is positioned in the active site loop of the thioredoxin protein (trxA), inside the dispensable region of the bacterial flagellin gene (fliC). The resultant recombinant fusion protein (FLITRX) is exported and assembled into partially functional flagella on the bacterial cell surface. The FliTrx library, which expresses random dodecapeptides flanked by cysteine-glycine-proline/glycine-proline-cysteine sequences (CGP/GPC) on the cell surface as FliC-Trx fusions.

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