BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: X15 E. coli bacterial display library

Library ID:

325

Library Length:

Library Complexity:

5.0e10

Library Titer:

Library From:

Patrick S. Daugherty

Library Randomness:

Completely random

Library Scheme:

NNS

Library Topology:

Linear

Comments:

E.coli OmpA was chosen as a display scaffold. To maximize library construction efficiency, asymmetric SfiI restriction sites were introduced into an OmpA expression vector immediately preceding loop 1 and following loop 4. DNA fragments containing the random epitope insertions were synthesized by polymerase chain reaction (PCR), digested with SfiI, ligated into the display vector and transformed into the E.coli strain MC1061, which can be made highly transformation competent and is ara(-), allowing the use of the araBAD promoter for controlled OmpA expression.

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