BDB - Target Details

BiopanningDataSet
Target
Template
Library
Structure

Target

Target Name: SH3 domain

Target ID:

1960

Target Type:

Protein>Others

Target Sequence:

Synonyms:

Target Source:

Homo sapiens (Human):9606

Target Structure:

Comments:

To conduct a large-scale analysis of human SH3 domain binding specificities, we identified 320 SH3 domains within 219 human proteins from an InterPro scan of the Ensembl database. We cloned genes encoding the 320 SH3 domains fused to the C-terminus of glutathione S-transferase(GST). In a high-throughput manner, we expressed the GST-SH3 fusions in Escherichia coli and successfully purified 215 GST-SH3 domains as determined by the presence of bands of the correct size by SDS-PAGE.

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