BDB - Target Details

Target

Target Name: Peptide KEQWFGNRWHEGYR

Target ID:

1959

Target Type:

Protein>Others

Target Sequence:

Synonyms:

Target Source:

None

Target Structure:

Comments:

Peptide KEQWFGNRWHEGYR encompassed a 14 amino acid of v6 domain of human CD44 protein, which was synthesized using an Advanced Chem Tech 396 multiple peptide synthesizer (Advanced ChemTech, Louisville, KY) using solid-phase Fmoc chemistry. A linker amino acid sequence of Gly-Ser-Gly (GSG) was inserted between the biotin and the NH2 terminus of the peptide to minimize any steric or chemical effects. Peptides were purified by reverse-phase highpressure liquid chromatography (RP-HPLC) on a C18 column (218TP54, Vydac, Hesparia, CA), lyophilized, and stored at -20 °C. Purity of the peptides was greater than 90% as assessed by HPLC. Identities of the peptides were confirmed by electrospray ionization mass spectrometry (Mass Consortium, San Diego, CA) at the Structure Core of University of Missouri, Columbia.

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