BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 962

Peptides:

Sequences

Affinity Values

KPWWVSRV
KPWYVGRP
KPWWVTRV
KPWWVVRL
GKPIWAGR
QKPIWVTR
DWAVWNRR
NWAVWTKR
NWGMWSKR
YVFEDLFR
MPEDFYRR
SEPVDHGL
VDPVDHGL
VPIDHGT

[0.97]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8170958

Target Name:

Purified IgM lamda receptor of the human Burkitt lymphoma cell line SUP-B8

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

8-mer P4-pIll and 8-mer P6-pIII phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Biotinylated peptide monomer (0.4 μg per well) was immobilized on a microtiter well coated with 1 μg of streptavidin per well. SUP-B8 immunoglobulin or control immunoglobulin (0.25 μg) was added. Optical density values, OD (405-490 nm), were measured. Data shown were reproduced from the graph and expressed as means of duplicate samples ± SD.

Brief Description:

BiopanningDataSet Comments:

All the peptides bound to the SUP-B8 immunoglobulin specifically. Their binding could be inhibited with anti-Id antibodies but not with anti-IgM or anti-lamda light-chain antisera. When conjugated to form dimers or tetramers, they induced cell death by apoptosis in vitro with an IC5o between 40 and 200 nM. This effect was associated with specific stimulation of intracellular protein tyrosine phosphorylation.

Curator:

Curation Date:

0000-00-00

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