BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 853

Peptides:

Sequences
SLSRMPIIGTSA(4) AWTHTLIRLPD(2) NEMTLIRMNMAA(2) DIRQPIIGTLHP(1) HIARTPIAGTNL(1) HRVPISGTSA(1) LEPTLIRLPQTL(1) MKETHSTLHQP(1)

Sequences Features:

Motif:

TLIR

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10880841

Target Name:

Anti-peptide (MSESHVKISRTIIRGTSPST) polyclonal antibody IgG

Template Name:

AA1–20 of Taenia solium paramyosin (MSESHVKISRTIIRGTSPST)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Peptide sequences was selected on panning of the 12-mer library in solution. A total of 300 ng of purified IgG fraction were mixed with the library (2e10 phage virions) and incubated 20min at room temperature. Then 50 ml of protein G-Agarose suspension (Gibco BRL) were added and the incubation was continued 15 min at the same temperature. The incubation mixture was centrifuged, supernatant discarded and the pellet washed 10 times with PBS-T. The final pellet was resuspended in 1 ml of glycine-HCl, pH 2.2 and after 10 min incubation at room temperature the eluate was collected after centrifugation The phage were titered, amplified and the selection was repeated two more times, first with 100 ng and then with 30 ng of IgG except for the third round where phage were eluted in two steps, first at pH 4.0 and then at pH 2.2.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

853

| Next

| Last