BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 852

Peptides:

Sequences
FRTAISGTPQFY(6) SYRTPITGTLIT(2) HSHTHKALAGTP(1) LVAKPHMRTPNL(1) WHWQYTPWWRGS(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10880841

Target Name:

Rabbit hyperimmune serum against peptide (MSESHVKISRTIIRGTSPST)

Template Name:

AA1–20 of Taenia solium paramyosin (MSESHVKISRTIIRGTSPST)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Rabbit hyperimmune serum, diluted 1:50 in PBS to give a final volume 300 ml, was distributed in 6 wells (50 ml:well) of 96-well flat bottom polystyrene microtitration plates.

BiopanningDataSet Comments:

Eight clones presented two sequences with internal RT-I-GT motif and three peptides contained only a part of this motif: RT or GT/S, or T-RGS. Comparison with the amino-terminal 20aa sequence of the TPmy highlighted the Arg10-Thr16 region of apparent homology to the motif shared by two of these peptides and partially by three of them.

Curator:

Curation Date:

0000-00-00

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