BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 789

Peptides:

Sequences

Affinity Values

MRKSTGTAS(2)
QKKMVALSG(1)
SRKVALQGG(1)
RRKLRTNAG(1)
RKRTRHLEG(1)
TQDLGYSRV(1)
RKKKPPGLA(1)
TRKALTQNT(1)
NRKLKSNMM(1)
KRRASAGGP(1)
RKVVQTSLE(1)
KGKKALQSA(1)
VRKLTVTSA(1)
KKTKGLVTT(1)
KSVKPRQAT(1)
FRKADKFPM(1)
RKTALGTRQ(1)
SRKSGSLTK(1)
KKKKYAEAV(1)
ARLSVVRNG(1)
SRKATASLP(1)
TRGLKGAPQ(1)
RKLAHPGTS(1)
GGTTTPRTT(1)
NRRLLPMPE(1)

[0.461/0.473]
[0.733]
[0.639]
[0.631]
[0.562]
[0.561]
[0.554]
[0.541]
[0.523]
[0.491]
[0.476]
[0.467]
[0.446]
[0.435]
[0.434]
[0.434]
[0.426]
[0.425]
[0.400]
[0.370]
[0.367]
[0.320]
[0.308]
[0.294]
[0.290]

Sequences Features:

Motif:

V-A-L-x-G

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3/5

Reference:

PMID: 11034389

Target Name:

Anti-GAD65 monoclonal antibody MICA4

Template Name:

Glutamate decarboxylase 2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pVIII-9aa.Cys phage display library (CX9C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The reactivity of phagotopes expressing that peptide with MICA4, as determined by capture ELISA, is given as an OD value measured at 405 nm.

Brief Description:

After each round of positive selection, there was a negative selection step in which phage not specifically reactive with the mAbs were removed using magnetic beads coated with anti-human Ig in the absence of primary Ab.

BiopanningDataSet Comments:

Twelve of 14 phage from the fifth round of positive selection and 14 of 21 phage from the third round of positive selection reacted by capture ELISA.

Curator:

Curation Date:

0000-00-00

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