BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 788

Peptides:

Sequences

Affinity Values

QKRAKGLSA(8)
YRKKSSAEL(2)
MRKANSPPT(2)
ARKVKGAAG(2)
RGLKSKIAM(1)
FRKTSISGA(1)
LSRKKTLTT(1)
RSLKTKLPS(1)
LRKKGYDPG(1)
SRKKTFTGA(1)
TKLSNTPSP(1)
GRKSAVSPA(1)
HRKSTVAGS(1)
FRKTSKGGS(1)
HKKSLSSPS(1)
RRKPGTEQL(1)
YRKTAKEGT(1)
YKKGARPIQ(1)

[0.441 ± 0.060]
[0.496/0.363]
[0.491/0.401]
[0.404/0.384]
[0.466]
[0.421]
[0.421]
[0.402]
[0.400]
[0.400]
[0.396]
[0.358]
[0.345]
[0.341]
[0.338]
[0.332]
[0.323]
[0.323]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3/5

Reference:

PMID: 11034389

Target Name:

Anti-GAD65 monoclonal antibody MICA3

Template Name:

Glutamate decarboxylase 2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pVIII-9aa.Cys phage display library (CX9C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The reactivity of phagotopes expressing that peptide with MICA3, as determined by capture ELISA, is given as an OD value measured at 405 nm.

Brief Description:

After each round of positive selection, there was a negative selection step in which phage not specifically reactive with the mAbs were removed using magnetic beads coated with anti-human Ig in the absence of primary Ab.

BiopanningDataSet Comments:

Twenty-eight of 35 phagotopes from the fifth round of positive selection and 0 of 32 phage from the third round of positive selection were reactive by capture ELISA.

Curator:

Curation Date:

0000-00-00

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