BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 730

Peptides:

Sequences

Affinity Values

VGSLR
RQTND
NQRSS
LQRAI
QRLRD
PDRHM
LSGGR
TVDYA
LSRDN
RGKTN
NNKLR
MQVKH
TTDLR
RVTST
AYGYK
STKGI
KLKET
VGLYD
VVMKD
RVDTG
GHRIN
YQSLN
SDKVY
HETLK
TSYLN
MQATK
EAPAK
PVHLY
QPNGY
AYGLA
YQNSS
SAVRP

[481000]
[415000]
[388000]
[367000]
[307000]
[243000]
[207000]
[134000]
[127000]
[80000]
[74000]
[34000]
[27000]
[26000]
[24000]
[20000]
[19000]
[18000]
[15000]
[15000]
[12000]
[12000]
[9000]
[9000]
[9000]
[8000]
[8000]
[7000]
[6000]
[6000]
[6000]
[5000]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 15843175

Target Name:

Kallikrein-14, hK14

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Substrate phage display library based on a modified pH0508b phagemid

Affinity Measurement Method:

Fluorescence

Affinity Measurement Description:

The CFP fluorescent substrate assay has been utilized to determine the kinetics of peptide substrate selection from a phage display library. The kinetic parameter kcat/Km (1/M × 1/s) was determined and shown.

Brief Description:

In fact, the target is recombinant hK14.

BiopanningDataSet Comments:

A SwissProt database search with selected sequences identified six potential human protein substrates for hK14. Two of them, laminin α-5 and collagen IV, which are major components of the extracellular matrix, have been demonstrated to be hydrolyzed efficiently by hK14.

Curator:

Curation Date:

0000-00-00

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