BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 69

Peptides:

Sequences	Affinity Values
CELYENVGMYC(18)	[0.49, 10-25]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9360978

Target Name:

Growth factor receptor-bound protein 2

Template Name:

Receptor tyrosine-protein kinase erbB-2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX9C phage display library

Affinity Measurement Method:

ELISA,Surface plasmon resonance (SPR)

Affinity Measurement Description:

The G1 phage is attached to the plate surface and GST-Grb2 domain is allowed to bind at 3 M of protein concentration. Binding was detected using anti-GST antibodies. Absorbance at 405 nm was measured. Data shown were reproduced from the graph. Besides, the IC50 values are determined by mixing peptide with recombinant Grb2 or Grb2 SH2 domains and then measuring the amount of binding at equilibrium to the immobilized SHC phosphopeptide, biotin-DDPSpYVNVQ. Synthetic G1 peptide blocks Grb2 SH2 domain association (IC50 10–25 μM) with the SHC phosphopeptide.

Brief Description:

In fact, the recombinant GST-Grb2 protein is target.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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