BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 658

Peptides:

Sequences
DVVYALSDD(14) AEYGESVNA(12) AEYGERGNA(6) AEYGESGNA(5) EGLVWIGMD(3) EAAGANIAP(2) AEYGESVLI(1) GAYDVNVND(1) DSDVGWVND(1) GPNWAEGDS(1) VADDRDYSD(1) VDVSEQMSL(1) VGDNVDYMD(1) VGDYDVVDS(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20185523

Target Name:

Prostate carcinoma cell line PC3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f8-9mer phage display library (X9)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The naive library was sequentially exposed to culture flasks, serum treated culture flasks and non-target cells (fibroblasts) before being incubated with target PC3 cells. The sequenced phage clones were from eluate, post-elution wash and lysate.

BiopanningDataSet Comments:

DVVYALSDD demonstrated specificity and selectivity to PC3 cells based on target-association assays, microscopy and flow cytometry.

Curator:

Curation Date:

0000-00-00

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