BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 657

Peptides:

Sequences

DTPYDLTG(15)
DTDSHVNL(9)
ERAPLSVE(6)
GDNSHVNL(5)
VGQDSDYS(4)
VSDNTDYS(3)
GPDSTWAG(2)
EGMMYTDV(2)
DLTYVNSQ(2)
DSSNKPTG(1)
DSSRLERV(1)
AHALTTEE(1)
APLPTNGE(1)
ATDHAAPQ(1)
ATPTTPDP(1)
EKFASNST(1)
EVSMYTDV(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20185523

Target Name:

Prostate carcinoma cell line PC3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f8-8mer phage display library (X8)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The naive library was sequentially exposed to culture flasks, serum treated culture flasks and non-target cells (fibroblasts) before being incubated with target PC3 cells. The sequenced phage clones were from eluate, post-elution wash and lysate.

BiopanningDataSet Comments:

DTDSHVNL and DTPYDLTG demonstrated specificity and selectivity to PC3 cells based on target-association assays, microscopy and flow cytometry.

Curator:

Curation Date:

0000-00-00

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