BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 581

Peptides:

Sequences
NHNMHRTTQWPL(6) YTPQKKIERAFG(2) YPHYSLPGSSTL(1) HWKHEMYPRTRL(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19400768

Target Name:

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Phages with encoded peptides were eluted with 100 μl of 1 mM meso-A2pm. NHNMHRTTQWPL inhibited MurE ATPase activity with an IC50 value of 500 μM. The inhibition was shown to be time-dependent and was reversed by the addition of meso-A2pm or UDP-MurNAc-Ala-Glu during the pre-incubation step. MurEp1 was found to interfere in meso-A2pm and UDP-MurNAc-Ala-Glu binding necessary for amide bond formation.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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