BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 580

Peptides:

Sequences
KVTLHHPPITRS(2) YTPQKKIERAFG(1) KSASNHQAHWLK(1) TPWHFHSTNGFR(1) INKPFHKVMPYA(1) HSVHSKARHLYT(1) TGLPLYINEGRP(1) YTTSNTLQVIAR(1) SFSNLAPSTRGT(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19400768

Target Name:

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Phages with encoded peptides were eluted with 100 μl of 1mM ATP. NHNMHRTTQWPL inhibited MurE ATPase activity with an IC50 value of 500 μM. The inhibition was shown to be time-dependent and was reversed by the addition of meso-A2pm or UDP-MurNAc-Ala-Glu during the pre-incubation step. MurEp1 was found to interfere in meso-A2pm and UDP-MurNAc-Ala-Glu binding necessary for amide bond formation.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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