BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 528

Peptides:

Sequences
CAWYEWPC(12) CAWYQFPC(6) CVWWQWPC(1) CPWFQWPC(1) CKWFQWPC(7) CFWVNTDC(1) CLYLSIRC(1)

Sequences Features:

Motif:

x-W-[FYW]-[QE]-[WF]-P

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18957413

Target Name:

Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a

Template Name:

Fc domain of IgG (IGHG1,IGHG2,IGHG3,IGHG4)

Structure of Target-Template Complex:

3RY6

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Cys6 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For the first round, FcγRIIA-R134-GST immoblized by precoated with goat anti-GST polyclonal antibody was used for panning. For other rounds, MaxiSorp strips coated FcγRIIA-R134-GST was used.

BiopanningDataSet Comments:

Among 29 selected clones, seven different amino acid sequences were found.

Curator:

Curation Date:

0000-00-00

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