BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 408

Peptides:

Sequences
WTITKHP(8) WRQTRKD(5) HAHTIST(5) KPVQLDH(3) HKRPRNN(2) ERYMGLP(2) IQLHPQH(1) SLDALLS(1) HAIYPRH(1) LVGNYTP(1) SILPYPY(1) TAPHPVL(1) HWRHNHS(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18221017

Target Name:

Arabidopsis Fip1 ortholog encoded by At5g58040 (AtFip1[V])

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Selection were carried out using the N-terminal 137 amino acids of AtFip1[V] as a target. A search of the Arabidopsis proteome using these Fip1-binding peptides as queries resulted in the identification of a number of putative Fip1-interacting proteins. One of these was the polyadenylation factor subunit, CstF77. This purported interaction was confirmed by yeast two-hybrid and in vitro assays. Mutation of the motif identified in the phage display screen eliminated the interaction, corroborating the results of the phage display screen.

BiopanningDataSet Comments:

Thirty-two clones were sequenced.

Curator:

Curation Date:

0000-00-00

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