BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 407

Peptides:

Sequences

WRQTRKD(18)
WHWRLPS(7)
HAIYPRH(6)
LRQTRKD(4)
LPWHKHR(4)
FHRHHNH(4)
HFFHRHT(3)
HWRHNHS(3)
WPFHHHR(3)
WHHKHRS(3)
WPWHKHS(2)
HKRPRNN(2)
HFTTRQR(2)
MPHIHRH(2)
PRRESRF(2)
KPAPRVH(2)
KHTVTRR(2)
HKTEHPA(1)
HRSPHTP(1)
SWRHHHH(1)
SYSNWTP(1)
QPPIYSA(1)
QPPNYNA(1)
KPRAGSF(1)
KGPHTQV(1)
IASGNSL(1)
IPTLPSS(1)
PTHRHRT(1)
TAPGVST(1)
LGPHTQV(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18221017

Target Name:

Arabidopsis Fip1 ortholog encoded by At5g58040 (AtFip1[V])

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Selection were carried out using the N-terminal 137 amino acids of AtFip1[V] as a target. A search of the Arabidopsis proteome using these Fip1-binding peptides as queries resulted in the identification of a number of putative Fip1-interacting proteins. One of these was the polyadenylation factor subunit, CstF77. This purported interaction was confirmed by yeast two-hybrid and in vitro assays. Mutation of the motif identified in the phage display screen eliminated the interaction, corroborating the results of the phage display screen.

BiopanningDataSet Comments:

Eighty-two clones were sequenced.

Curator:

Curation Date:

0000-00-00

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