BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3676

Peptides:

Sequences
GLHTSATNLYLH(2) SLDGAGAALRTS(2) DRWVARDPASIF(1) GDGNSVLKPGNW(1) HTPMSSRLSTAS(1) SGVYKVAYDWQH(1) SKGDSLPFPFAT(1) SNSIDKVNRPIN(1) VVSPDMNLLLTN(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 36746976

Target Name:

Vitamin D binding protein, VDBP

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Data are expressed as the means of the absorbance value at (410 ± SD) nm.

Brief Description:

One point five mL of phage pool diluted with TBST (TBS + 0.1%.v/v Tween-20) to 1.0e9 Plaque Forming Units per mL (pfu/mL) from the phage library was attached to the petri dish (polystyrene). Then, the solution was rocked at room temperature (RT) for 45 min to remove phages attached to the petri dish (polystyrene) first. The BSA-coated plate was washed 5 times with TBST. Then, the supernatant of the phage pool reacted with a petri dish (polystyrene) and was recovered and put into the BSA-coated plate. Also, it was bound by rocking at room temperature (RT) for 45 min. After that, unbound phages were collected and used for main biopanning.

BiopanningDataSet Comments:

Curator:

Bowen Li

Curation Date:

2023-10-13

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