BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3663

Peptides:

Sequences	Affinity Values
DHPRFNDSYNSP DHPRFNRDNDVA DHPRFNYVSQPW	[1.2744±0.1438] [1.3154±0.0913] [1.1443±0.089]

Sequences Features:

Motif:

DHPR

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 37038893

Target Name:

Anti-Ara h 2 IgG

Template Name:

Conglutin-7

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance was measured at 450 nm with a microtiter plate reader. The value of OD450 was reproduced from Figure S1A and shown.

Brief Description:

Bound phages were eluted either specifically with Ara h 2 or non-specifically with 0.1 M glycine buffer with pH 2.2.

BiopanningDataSet Comments:

We identified and selected three linear peptides (DHPRFNRDNDVA, DHPRYGP and DHPRFST), and immunoblot analyses revealed binding to lgE from peanut-allergic individuals. The peptide sequences were aligned to the disordered region corresponding to the loop between helices 2 and 3 of Ara h 2, and conformational mapping showed that the peptides match the surface of Ara h 2 and h 6 but not other peanut allergens. In ImmunoCAP inhibition experiments, the peptides significantly inhibit the binding of IgE to Ara h 2 (p < .001). In basophil and mast cell activation tests, the peptides significantly suppressed Ara h 2-induced effector cell activation (p < .05) and increased the half-maximal Ara h 2 effective concentration (p < .05). Binding of the peptides to specific IgEs did not induce activation of basophils or mast cells.

Curator:

Bowen Li

Curation Date:

2023-10-13

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