BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3662

Peptides:

Sequences	Affinity Values
CLDLNPYYC CNDYFPKLC CGDFWPRLC	[1.2211] [0.8144] [1.2191]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26908079

Target Name:

Anti-Fel d 1 IgG

Template Name:

Major allergen I polypeptide chain 1 and 2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance was measured at 450 nm with a microtiter plate reader. The ratio of the ELISA signal of the test phage wells to the wild-type phage (control phage) wells was calculated. The ratio value was reproduced from Figure 1 and shown.

Brief Description:

Bound phages were eluted with either rFel d 1 or with 0.1 M glycine buffer with pH 2.2.

BiopanningDataSet Comments:

The mimotopes were computationally mapped to the allergen surface, and their IgE reactivity was confirmed using sera from cat-allergic patients. Importantly, the mimotopes showed nobasophil activation of the corresponding cat-allergic patients, which makes them good candidates forthe development of hypoallergenic vaccine. As bacteriophage particles are becoming increasingly recognized as immunogenic carriers, we constructed bacteriophage particles displaying multiple copies of each selected mimotope on major phage coat protein. These constructed phages elicited T cell-mediated immune response, which was predominated by the type 1 T cell response. Mimotopes alone contributed to the type 1 T cell response by promoting IL-2 production. Fel d 1 mimotopes, as well as their filamentous phage immunogenic carriers, represent promising candidates in the development of hypoallergenicvaccine against cat allergy.

Curator:

Bowen Li

Curation Date:

2023-10-13

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