BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3660

Peptides:

Sequences

Affinity Values

CWTDLGRKC
CWDSLGRAC
CWDAMGRAC
CVPDWQGRC
CIPNNLGRC
CIPNPIGRC
CYNTIGRSC
CNDKSKPYC
CLDKSKPVC
CLDKSKPQC
CVDKSKPHC
CVDRSKPTC
CKDRSKPDC
CPDRSKPHC
CYDRSKPYC
CDHSKPHTC
CDRSKPLHC

[2.239±0.0655]
[1.4224±0.0756]
[1.6442±0.1059]
[2.0374±0.121]
[1.251±0.0907]
[1.4325±0.1865]
[0.9335±0.0807]
[1.4325±0.121]
[1.9214±0.1159]
[1.6694±0.1462]
[2.1432±0.1059]
[1.7248±0.0504]
[2.1987±0.1159]
[1.8811±0.0907]
[1.871±0.0756]
[0.631±0.0554]
[0.2933±0.0907]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30315830

Target Name:

Anti-Api m 1 IgG

Template Name:

Phospholipase A2, bvPLA2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance was measured at 450 nm with a microtiter plate reader. A(450 nm) were reproduced from Figure E1A and shown.

Brief Description:

Bound phages were eluted from the target antibodies either with 0.1 mol/L glycine-HCl (pH 2.2) for 10 minutes followed by immediate neutralization with 1 mol Tris (pH 8.0) or competitively with Api m 1 at the final concentration of 12 mg/mL.

BiopanningDataSet Comments:

Curator:

Bowen Li

Curation Date:

2023-10-13

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